Journal: Cell Reports Methods

Article Title: SynBot is an open-source image analysis software for automated quantification of synapses

doi: 10.1016/j.crmeth.2024.100861

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Article Snippet: Mouse anti-neural cell adhesion molecule L1 Hybridoma , Developmental Studies Hybridoma Bank , Cat# ASCS4 RRID: AB_528349.

Techniques: Recombinant, Plasmid Preparation, Electron Microscopy, Bicinchoninic Acid Protein Assay, Microscopy, Software, Low Protein Binding

Journal: Communications Biology

Article Title: Abnormal cytoskeletal remodeling but normal neuronal excitability in a mouse model of the recurrent developmental and epileptic encephalopathy-susceptibility KCNB1-p.R312H variant

doi: 10.1038/s42003-024-07344-6

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Article Snippet: Anti-neural cell adhesion molecule L1 antibody, clone 324 , Millipore Sigma , Cat# MAB5272 RRID:AB_2133200.

Techniques: Plasmid Preparation, MTT Assay, In Vivo, Isolation, Recombinant, Software

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: (A) Left: canonical RNA regulation by the exon junction complex (EJC) composed of core components EIF4A3, RBM8A, and MAGOH. Right: the question addressed in this study. How do EJC components control neuronal development?. (B) Coronal section from a control ( Eif4a3 lox/lox ) E17.5 mouse cortex, stained with L1CAM and Hoechst. (C) Higher-magnification images from the region indicated by the dashed box in (B), stained with L1CAM and CC3. White bracket, cortical axonal tract thickness. Arrow, region with apoptotic nuclei. (D) Fold change of L1CAM+ axonal thickness in E17.5 Magoh, Rbm8a, and Eif4a3 cHet and cKO cortices relative to control ( Magoh lox/lox , Rbm8a lox/lox and Eif4a3 lox/lox , respectively). ** p = 0.0065, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 3–6 embryos. (E) CC3+ cell density in E17.5 Magoh, Rbm8a, and Eif4a3 cHet and cKO relative to control ( Magoh lox/lox , Rbm8a lox/lox , and Eif4a3 lox/lox , respectively). ** p < 0.01, *** p < 0.001, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 3 embryos. (F) Coronal sections of control ( Eif4a3 lox/lox ; p53 lox/lox ) and Eif4a3; p53 dcKO E17.5 mouse cortices, stained with L1CAM, CC3, and Hoechst. White bracket, cortical axonal tract thickness. (G) Fold change of L1CAM+ cortical axonal thickness in E17.5 Eif4a3, p53 dcHet, and dcKO relative to control ( Eif4a3 lox/lox ; p53 lox/lox ). ** p = 0.0099, one-way ANOVA (Dunnett’s multiple-comparisons test), n = 4 embryos. (H) Fold change of L1CAM+ fibers/cortical thickness ratio from E17.5 Eif4a3 cKO relative to control ( Eif4a3 lox/lox ) and on a p53 background. * p = 0.0491, *** p < 0.001, one-way ANOVA (Dunnett’s multiple-comparison test), n = 6 embryos. All graphs, mean + SD; ns, not significant. Scale bars: 500 μm (B), 100 μm (C), and 50 μm (F). See also and .

Article Snippet: Rat monoclonal anti-Neural Cell Adhesion Molecule L1 (L1CAM) (clone 324) , Millipore , Cat#MAB5272; RRID:AB_2133200.

Techniques: Control, Staining, Comparison

Journal: Cell reports

Article Title: The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton

doi: 10.1016/j.celrep.2024.114666

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rat monoclonal anti-Neural Cell Adhesion Molecule L1 (L1CAM) (clone 324) , Millipore , Cat#MAB5272; RRID:AB_2133200.

Techniques: Virus, Recombinant, Electron Microscopy, Protease Inhibitor, Binding Assay, Spin Down Assay, Control, Plasmid Preparation, Software